Thermo Scientific™

Phusion U Green Hot Start DNA Polymerase

Catalog number: F556S
Thermo Scientific™

Phusion U Green Hot Start DNA Polymerase

Catalog number: F556S
Catalog Number
F556S
also known as F-556S
Unit Size
100 units
Price (USD)
189.00
Your Price:
Availability
***
Quantity
Catalog Number
F556L
also known as F-556L
Unit Size
500 units
Price (USD)
736.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F556S
also known as F-556S
100 units
189.00
Your Price:
***
F556L
also known as F-556L
500 units
736.00
Your Price:
***
Product Overview
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Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)
Green format—permits direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
PCR Amplification
Product Line
Phusion
Product Type
Hot Start DNA Polymerase
Quantity
100 Units
Concentration
2 U/µL
Fidelity (vs. Taq)
25 X
Form
Gel
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
Overhang
Blunt
Polymerase
Phusion U Hot Start DNA Polymerase
Reaction Format
Standalone
Reaction Speed
Fast
Size (Final Product)
20 kb

Contents & Storage

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.

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Frequently asked questions (FAQs)

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