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Catalog Number | Color | No. of Reactions |
---|---|---|
F564L | Green | 500 Reactions |
F564S | Green | 100 Reactions |
F562L | Colorless | 500 Reactions |
F562S | Colorless | 100 Reactions |
The Phusion U Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Green format further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.
Highlights
Applications
The master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mixes can even be used with unpurified samples such as blood or serum.
Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.
Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).