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Catalog Number | Color | No. of Reactions |
---|---|---|
F566L | Green | 500 Reactions |
F566S | Green | 100 Reactions |
F565L | Colorless | 500 Reactions |
F565S | Colorless | 100 Reactions |
Highlights
With Phusion Hot Start II High-Fidelity DNA Polymerases amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3'→5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.
Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Applications
Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).