Canine Genotypes Panel 1.1 Kit

Catalog number: F860L

Thermo Scientific™  Related applications: Animal Health | PCR

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Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.

The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks". One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).


• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
For Research Use Only. Not for use in diagnostic procedures.


Technique: STR (Short Tandem Repeat) Analysis
Product Size: 500 reactions
Detection Method: Capillary Electrophoresis
Genotyping Target: Canine Genotyping and Parentage Testing

Contents & storage

• 5 x 1.1 mL Canine Genotypes Panel 1.1 Master Mix
• 5 x 1.1 mL Canine Genotypes Panel 1.1 Primer Mix
• 150 µL Canine Genotypes Control DNA001

Store at -20°C. Primer Mix should be protected from light at all the times.


Manuals & protocols