Canine Genotypes Panel 2.1 Kit

Catalog number: F864S

Thermo Scientific™  Related applications: Animal Health | PCR

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Description

Thermo Scientific Canine Genotypes Panel 2.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.

The Canine Genotypes Panel 2.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 2.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks". One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

Canine Genotypes Panel 2.1 is a kit for canine forensics, identification, parentage and breed testing. It encompasses the following 19 loci: PEZ02, PEZ17, FH2017, FH2309, PEZ05, FH2001, FH2328, FH2004, FH2361, PEZ21, FH2054, FH3377, FH2107, FH2088, vWF.X, FH2010, PEZ16 FH3313 and ZFX/Y (sex determining locus). These markers underwent a large developmental and validation study and were standardized for use in forensics (see references).

Highlights

• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Capillary Electrophoresis
Genotyping Target: Canine Genotyping and Parentage Testing, Canine forensics
Product Size: 100 reactions
Technique: STR (Short Tandem Repeat) Analysis

Contents & storage

Contains:
• 1.1 mL Canine Genotypes Panel 2.1 Master Mix
• 1.1 mL Canine Genotypes Panel 2.1 Primer Mix
• 30 µL Canine Genotypes 2.1 Control DNA

Store at -20°C. Primer Mix should be protected from light at all the times.

Documents

Manuals & protocols