Perfluorohexane vapor has only minor effects on spatial pulmonary blood flow distribution in isolated rabbit lungs.
AuthorsHübler M, Heller AR, Bleyl JU, de Abreu MG, Kroll T, Rössel T, Koch T
JournalAnesth Analg
PubMed ID15781532
'We tested the hypothesis that administration of perfluorohexane (PFH) vapor does not significantly affect the relative pulmonary blood flow (Qrel) distribution in isolated rabbit lungs. Fourteen isolated rabbit lungs were perfused with a Krebs-Henseleit buffer solution (flow 150 mL/min). Pulmonary afterload was set to 3 mm Hg. The lungs were ... More
Validation of fluorescent-labeled microspheres for measurement of regional organ perfusion.
AuthorsGlenny RW, Bernard S, Brinkley M
JournalJ Appl Physiol
PubMed ID8335595
'Estimations of dog lung, pig heart, and pig kidney regional perfusion by use of fluorescent-labeled microspheres were compared with measurements obtained with standard radiolabeled microspheres. Pairs of radio- and fluorescent-labeled microspheres (15 microns diam, 6 colors) were injected into a central vein of a supine anesthetized dog and the left ... More
Fibroblast growth factor 4 induces vascular permeability, angiogenesis and arteriogenesis in a rabbit hindlimb ischemia model.
AuthorsRissanen TT, Markkanen JE, Arve K, Rutanen J, Kettunen MI, Vajanto I, Jauhiainen S, Cashion L, Gruchala M, Närvänen O, Taipale P, Kauppinen RA, Rubanyi GM, Ylä-Herttuala S
JournalFASEB J
PubMed ID12475908
'Previous studies have shown that fibroblast growth factor (FGF)-1, FGF-2, and FGF-5 induce therapeutic angiogenesis. Here, we investigated the potential of FGF-4 for therapeutic neovascularization in comparison to vascular endothelial growth factor (VEGF), using adenoviral gene transfer in a novel rabbit hind limb ischemia model, with ischemia restricted to the ... More
Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.
'The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, ... More
Measuring bone blood supply in mice using fluorescent microspheres.
AuthorsSerrat MA,
JournalNat Protoc
PubMed ID19893510
Fluorescent microspheres are commonly used to assess bone blood supply in large animals, but the technique is not widely used in smaller mammals, as traditional methods such as reference blood sampling, ventilation and catheterization are not easily applied. This protocol describes a viable alternative for measuring bone and organ perfusion ... More
Effects of gestational age and cortisol treatment on ovine fetal heart function in a novel biventricular Langendorff preparation.
AuthorsFletcher AJ, Forhead AJ, Fowden AL, Ford WR, Nathanielsz PW, Giussani DA,
JournalJ Physiol
PubMed ID15513943
Structural and functional maturation of a number of fetal organs and physiological systems occurs in the immediate period prior to term, in association with the prepartum increase in fetal plasma cortisol concentration. At present, little is known about how myocardial sensitivity to adrenergic and muscarinic cholinergic stimulation changes as the ... More
Multiplexed spectral signature detection for microfluidic color-coded bioparticle flow.
Here, we report a high-speed photospectral detection technique capable of discriminating subtle variations of spectral signature among fluorescently labeled cells and microspheres flowing in a microfluidic channel. The key component used in our study is a strain-tunable nanoimprinted grating microdevice coupled with a photomultiplier tube (PMT). The microdevice permits acquisition ... More
Development of a novel fluorescent microsphere technique to combine serial cerebral blood flow measurements with histology in the rat.
AuthorsDe Visscher G, Haseldonckx M, Flameng W, Borgers M, Reneman RS, van Rossem K
JournalJ Neurosci Methods
PubMed ID12573474
The aim of the study was to evaluate the microsphere technique for the quantitative assessment of regional cerebral blood flow (rCBF) at different time points in the same animal. Yellow-green and red fluorescent microspheres with a diameter of 15 microm were injected into the rat at two different time points ... More
Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique.
A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) ... More
Single-cell volume estimation by three-dimensional wide-field microscopy applied to astroglial primary cultures.
Astrocytes, which constitute a prominent part of the number and volume of brain cells, have a high capacity for controlling their volume, and astrocytic swelling is associated with a number of pathological states affecting the CNS. In order to understand the mechanisms for regulating cell volume in astrocytes better, it ... More
Characterization of retinal leukostasis and hemodynamics in insulin resistance and diabetes: role of oxidants and protein kinase-C activation.
AuthorsAbiko T, Abiko A, Clermont AC, Shoelson B, Horio N, Takahashi J, Adamis AP, King GL, Bursell SE
JournalDiabetes
PubMed ID12606527
Increases in leukostasis/monocyte adhesion to the capillary endothelium (leukostasis) and decreases in retinal blood flow may be causally associated and are implicated in the pathogenesis of diabetic retinopathy. In this study, we demonstrate that increases in leukostasis are observed in insulin-resistant states without diabetes, whereas decreases in retinal blood flow ... More