Invitrogen™

NP40 Cell Lysis Buffer

Catalog number:  FNN0021

Related applications:

Protein Assays and Analysis

| Protein Purification & Isolation

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Description

NP40 Cell Lysis Buffer is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications. The buffer is used for total protein extraction and utilizes detergent-based lysis, eliminating the need for mechanical cell disruption. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.

Buffer formulation: 50 mM Tris, pH 7.4, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3VO4, 1% Nonidet P40 (NP40), 0.02% NaN3

This cell lysis buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

View all available ELISA lysis buffers and reagents.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Cell Lysis Buffer
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C).