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Citas y referencias (8)
Invitrogen™
Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)
La adenosina 5'-trifosfato (A-12410) BODIPY FL y la guanosina 5'-trifosfato BODIPY FL se componen de un fluoróforo BODIPY FL fijadoMás información
| Número de catálogo | Cantidad |
|---|---|
| G12411 | 100 μl |
Número de catálogo G12411
Precio (MXN)
-
Cantidad:
100 μl
La adenosina 5'-trifosfato (A-12410) BODIPY FL y la guanosina 5'-trifosfato BODIPY FL se componen de un fluoróforo BODIPY FL fijado a la posición 2' o 3' del anillo de ribosa a través de un enlace. Las interacciones entre el fluoróforo y la base de purina son evidentes a partir de las propiedades espectroscópicas de estos análogos de nucleótidos. El rendimiento cuántico de fluorescencia de GTP BODIPY FL es solo aproximadamente una décima parte del de ATP BODIPY FL; sin embargo, este rendimiento puede mejorarse tras la unión de GTP BODIPY FL a proteínas de unión a GTP.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteBODIPY™ FL
Tipo de productoGuanosina 5'-trifosfato
Cantidad100 μl
Condiciones de envíoHielo húmedo
Concentración5 mM
Línea de productosBODIPY
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (8)
Citations & References
Abstract
Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.
Journal:Anal Chem
PubMed ID:17263349
'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product,
Automated single-cell electroporation.
Journal:Biotechniques
PubMed ID:17068953
Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.
Journal:J Biomol Screen
PubMed ID:20008126
Small GTPases are key regulators of cellular activity and represent novel targets for the treatment of human diseases using small-molecule inhibitors. The authors describe a multiplex, flow cytometry bead-based assay for the identification and characterization of inhibitors or activators of small GTPases. Six different glutathione-S-transferase (GST)-tagged small GTPases were bound
A direct fluorescence-based assay for RGS domain GTPase accelerating activity.
Journal:Anal Biochem
PubMed ID:15840508
Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide binding proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the Galpha subunit. RGS (regulator of
Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.
Journal:J Biol Chem
PubMed ID:15613467
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max