Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant.
For Research Use Only. Not for use in diagnostic procedures.
Excitation Wavelength Range
For Use With (Equipment)
Nucleus, Nucleic Acids
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
A zebrafish retina cryosection labeled with the mouse monoclonal antibody FRet 43 and detected using TSA Kit #2 with the HRP conjugate of goat anti–mouse IgG antibody and Alexa Fluor® 488 tyramide.