Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.
Cells per vial: 4-8 million
Hepatocyte cultures were incubated with radiolabeled taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP/BSEP and OATPs/MRP2. Accumulation rates and facilitated active uptake were calculated.
Get the Results You Need with GIBCO Hepatocytes High quality hepatocytes, with high viabilities, proper cell morphology, and transporter function increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for: • Viability ≥ 70% • Attachment efficiency • Monolayer formation and integrity • Confluency >80% on Day 4 • Phase I enzyme activities • Transporter activity • Bile canaliculi formation • Relevant CYP450 polymorphisms
For research use only. Not intended for human or animal therapeutic or diagnostic use.