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ImaGene Green™ C12FDGlcU GUS Gene Expression Kit

Catalog number: I2908

Molecular Probes™  Related applications: Cellular Imaging | Flow Cytometry | Protein Assays and Analysis

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Description

The ImaGene Green™ GUS Gene Expression Kit contains a fluorescein-based glucuronidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDGlcU. Once inside the cell, the substrates are cleaved by ß-glucoronidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDGlcU, the kits also include the broad-spectrum ß-glucoronidase inhibitor, D-glucaric acid-1,4-lactone.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: GUS (β-Glucuronidase) Assay
Product Size: 1 kit
Target Enzyme: Beta Glucuronidase (GUS)
Substrate Type: Beta-Glucuronidase (GUS) Substrate
Substrate Properties: Chemical Substrate
Excitation⁄Emission (nm): 495⁄518
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

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