Thermo Scientific™

Biocemical Identification System (O.B.I.S.) PYR

Catalog number: ID0580M
Thermo Scientific™

Biocemical Identification System (O.B.I.S.) PYR

Catalog number: ID0580M

Rapidly determine PYRase activity is streptococci and Citrobacter spp. with Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) PYR. This rapid colorimetric test delivers simple and rapid results for the determination of PYRase activity in streptococci and Citrobacter spp, offering a rapid diagnostic alternative to time-consuming culture methods. In addition, the same enzymatic activity may be used to aid differentiation of Salmonella spp. from Citrobacter spp and other Enterobacteriaceae1,2.

 
Catalog Number
ID0580M
Unit Size
Each
Quantity
60 Tests/Kit
Price (USD)
Full specifications
Quantity60 Tests/Kit
DescriptionO.B.I.S. PYR
Detectable AnalytesPYRase activity
IncludesTest cards, developing solution, buffer solution
Unit SizeEach
Showing 1 of 1
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)
ID0580MFull specifications
Each60 Tests/KitRequest A Quote
Quantity60 Tests/Kit
DescriptionO.B.I.S. PYR
Detectable AnalytesPYRase activity
IncludesTest cards, developing solution, buffer solution
Unit SizeEach
Showing 1 of 1

Use O.B.I.S. PYR as a rapid diagnostic alternative to time-consuming culture methods.

  • Simple—easy to implement
  • Rapid—results in 6 minutes of testing suspect colonies
  • Non-carcinogenic substrates

The O.B.I.S. - PYR test uses Test Cards impregnated with L-pyroglutamic acid 7-amino-4-methyl-coumarin (7AMC) and dimethylaminocinnamaldehyde for the detection of PYRase activity. The enzymatic hydrolysis of this substrate by enterococci, Group A streptococci and Citrobacter spp. produces a purple color following the addition of the Developing Solution3.

We have developed a new system for PYRase testing4 using a non-carcinogenic substrate in response to health concerns associated with the use of β-naphthylamide (a potent carcinogen)5. The PYRase area on the O.B.I.S. PYR test is impregnated with L-pyroglutamic acid 7-amino-4-methylcoumarin (7AMC)4. Dimethylaminocinnamaldehyde is used as a color development reagent. The enzymatic hydrolysis of the substrate produces a purple color on addition of the O.B.I.S. PYR Developing Solution4.

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Contents & Storage

2°C to 8°C

Figures

Frequently asked questions (FAQs)

Citations & References