Thermo Scientific™

UltiMate™ 3000 Rapid Separation Quaternary System

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Industrial & Applied Science

Get maximum application flexibility with the Thermo Scientific™ UltiMate™ 3000 Rapid Separation Quaternary System, which supports the use of up to four solvents at up to 15,000 psi (100 MPa, 1000 bar) for fast routine applications or method development.


With excellent flow stability, a large choice of detection technologies and a recommended flow rate range from 200µL/min to 8.0mL/min, it adapts easily for multiple users running diverse methods in routine environments as well as research and development laboratories. It is your most flexible system choice for established and novel liquid chromatography workflows.

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Recommended Use

The UltiMate 3000 Rapid Separation Quaternary system is an excellent choice for laboratories that want outstanding UHPLC technology but which still need to maintain established LC methods developed on quaternary systems. It is suitable for use with both ultra-high resolution and conventional columns with particles sizes ranging from sub-2μm to 10µm. It handles a range of separations from 2mm column internal diameters to small scale semi-prep or purification experiments.

Typical application areas are fast but highly resolving methods in drug development, academic research, high-throughput quality control, chemicals development and demanding mass spectrometry workflows. The UltiMate 3000 Rapid Separation Quaternary system is highly recommended for methods using either conventional 3-5µm or UHPLC, sub-2µm particle columns where moderately fast gradient response is acceptable for the benefit of improved mobile phase flexibility (i.e., multiple solvents, extended column wash, ternary or quaternary gradients and method development).

Ensuring Excellent Chromatography

  • Up to 8 mL/min flow rate supporting up to 100 Mpa (15000 psi/1000 bar) backpressure for column format and column particle size flexibility. Manage backpressure and fine tune resolution with column heating and cooling from 5°C up to 110°C (mobile phase preheater and post-column cooler included)
  • Obtain near-zero dead volume for optimal peak shapes even over extended periods using Thermo Scientific™ Dionex™ Viper™ Fingertight Fitting system (recommended)
  • Minimizes contamination between samples for more accurate results with the ultra-low carryover autosamplers (with loop and needle permanently in the flow path)
  • Get precise and accurate flow rates for stable retention times, robust peak integration, and confident identification from the Thermo Scientific™ Dionex™ SmartFlow™ technology
  • Lets you optimize mixing efficiency with adjustable gradient delay volumes using Thermo Scientific™ Dionex™ SpinFlow technology
  • Transfer methods between systems with confidence: the UltiMate Quaternary Rapid Separation System shares an identical system delay volume with the Quaternary Standard System by design

Chromeleon CDS Software Makes Operation Easy

  • Integrated service and validation monitoring with Thermo Scientific™ Dionex™ Chromeleon™ CDS, versions 6.x and 7.x
  • Automated system startup, standby and shutdown with Chromeleon CDS, versions 6.x and 7.x
  • Compatible with Chromeleon 7.x eWorkflow walk-up solution and advanced reporting features

Choose the Right Configuration for You

  • Four-solvent channel quaternary pump with integrated four-channel degasser (LPG-3400RS)
  • Spacious solvent rack for large mobile phase bottles (SR-3000)
  • Thermostatted or nonthermostatted autosamplers (WPS-3000TSL/WPS-3000SL)
  • Thermostatted column compartment with up to two field upgradable column switching valves, (TCC-3000RS)
  • Diode array, multiple wavelength, variable wavelength, fluorescence, charged aerosol, refractive index or mass spectrometry detectors
  • Fraction collector (AFC-3000)
  • Chromeleon 7.2 CDS software packages (standalone, networked, remote data processing and/or control)