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View additional product information for Alkaline Phosphatase, calf intestine, EIA Grade - FAQs (J61037.LB0, J61037.MCR)
11 product FAQs found
BioAcryl-P (30%, 37.5:1) is suitable for conjugation to antibodies and other proteins for ELISA, Western blotting, and other histochemical procedures. Alkaline phosphatase may be used to dephosphorylate the 5' ends of DNA or RNA. It can also be used to dephosphorylate proteins.
The specific activity of BioAcryl-P (30%, 37.5:1) is ≥3,000 units/mg protein (DEA units).
The Km is 1.5 x 10-3 M (p-Nitrophenyl phosphate); 19 x 10-3 M (phosphoenolypyruvate).
The extinction coefficient (278nm) of BioAcryl-P (30%, 37.5:1) is 7.6-10.5.
The molecular mass of BioAcryl-P (30%, 37.5:1) is ~ 140 kDa.
The EC for BioAcryl-P (30%, 37.5:1) is 3.1.3.1.
One unit is defined as the amount of protein that hydrolyzes 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C. One glycine unit is equal to ~3 DEA units.
Optimum pH varies depending on the substrate, substrate concentration, and the ionic concentration. The enzyme is most stable at a pH range of 7.5-9.5. The optimum pH range for catalytic activity is 8-10. The enzyme has optimal activity using Diethanolamine (DEA) as a substrate at a pH of ~9.8.
The enzyme activity can be inhibited by addition of chelating agents or inorganic phosphates.
Alkaline Phosphatase, calf intestine, EIA Grade is supplied as a 20 mg/mL protein solution in 50% glycerol, containing 5 mM Tris, 5 mM MgCl2 and 0.12 mM ZnCl2.
Alkaline Phosphatase, calf intestine, EIA Grade should be stored at -20°C.