BSA Blocking Buffer, 3% in PBS

Name: BSA Blocking Buffer, 3% in PBS
SKU: J61655.AK

BSA (bovine serum albumin) blocking buffer, 3% in PBS, is a blocking agent ideal for saturating excess protein-binding sites on membranes.

Change view

Quantity:

250 mL

500 mL

Try the chem dex^TM web app

This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.

Catalog number J61655.AK

also known as J61655-AK

Price (USD)

Quantity:

250 mL

Request bulk or custom format

Specifications

Chemical Name or MaterialBlocking Buffer

Recommended StorageStore at -20°C

Concentration3% in PBS

Physical FormLiquid

Quantity250 mL

BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• BSA blocking buffer contains a serum albumin protein isolated from a cow with a high affinity for proteins

Application

• BSA (bovine serum albumin) blocking buffer is used as a blocking reagent ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications
• This buffer can be used in a range from 0.3% to 5% dissolved in PBS solution

RUO – Research Use Only

General References:

Kalwa, H.; Storch, U.; Demleitner, J.; Fiedler, S.; Mayer, T.; Kannler, M.; Fahlbusch, M.; Barth, H.; Smrcka, A.; Hildebrandt, F.; Gudermann, T.; Dietrich, A. Phospholipase C Epsilon (PLCepsilon) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. J. Cell. Physiol. 2015, 230 (6), 1389-1399.
Jeong, M. S.; Ahn, D. R. A microwell plate-based multiplex immunoassay for simultaneous quantitation of antibodies to infectious viruses. Analyst 2015, 140 (6), 1995-2000.