RBC lysis buffer for mouse - FAQs

We recommend the following procedure:
1. Dilute 1 volume of cell suspension with 10 volumes of RBC lysis buffer for mouse.
2. Vortex for 5 sec and incubate for 10 min at room temperature. If the sample is not transparent red after 10 min, incubate for another 2 min. Stop reaction by adding 20-30 mL of 1X PBS.
3. Centrifuge at 300xg for 10 min at 4 degrees C. Aspirate supernatant. White blood cells are present in the pellet.
4. Repeat the lysis process if red blood cells are still present in the pellet.
5. Resuspend the cell pellet in an appropriate buffer and proceed for further applications.

This buffer is able to lyse mouse RBCs. It contains ammonium chloride that lyses the RBC and leaves the WBCs intact. The resulting WBCs can be used for staining or nucleic acid isolation.

RBC lysis buffer for mouse contains 10 mM Tris-HCl, 150 mM ammonium Chloride at pH 7.2-7.4.