BSA Blocking Buffer, 5% in PBS, with 0.05% Tween-20

Name: BSA Blocking Buffer, 5% in PBS, with 0.05% Tween-20
SKU: J63711.AK

BSA (bovine serum albumin) blocking buffer, 3% in PBS, is a blocking agent ideal for saturating excess protein-binding sites on membranes.

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Quantity:

250 mL

500 mL

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Catalog number J63711.AK

also known as J63711-AK

Price (USD)

Quantity:

250 mL

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Specifications

Chemical Name or MaterialBlocking Buffer

Recommended StorageStore at -20°C

Physical FormLiquid

Quantity250 mL

Unit SizeEach

Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the microwell plate or membrane.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

BSA blocking buffer contains a serum albumin protein isolated from a cow with a high affinity for proteins

Application

BSA (bovine serum albumin) blocking buffer is used as a blocking reagent ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications
This buffer can be used in a range from 0.3% to 5% dissolved in PBS solution

RUO – Research Use Only

General References:

Kalwa, H.; Storch, U.; Demleitner, J.; Fiedler, S.; Mayer, T.; Kannler, M.; Fahlbusch, M.; Barth, H.; Smrcka, A.; Hildebrandt, F.; Gudermann, T.; Dietrich, A. Phospholipase C Epsilon (PLCepsilon) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. J. Cell. Physiol. 2015, 230 (6), 1389-1399.
Jeong, M. S.; Ahn, D. R. A microwell plate-based multiplex immunoassay for simultaneous quantitation of antibodies to infectious viruses. Analyst 2015, 140 (6), 1995-2000.