Search Thermo Fisher Scientific
Element Search
Structure SearchThermo Scientific Chemicals
Albumin, Bovine, Cohn Fraction V, 98%, Cell Culture Grade, Low Endotoxin
Catalog number: J65097.18
50g, Each
Thermo Scientific Chemicals
Albumin, Bovine, Cohn Fraction V, 98%, Cell Culture Grade, Low Endotoxin
Catalog number: J65097.18
50g, Each
Quantity
Specifications
Specification SheetAssay (special)
? 98.0% (Protein on dry basis - as determined ny nitrogen analysis)
Assay (special)
Aerobic plate count: ? 1000 cfu/gm
Appearance (Color)
White to yellow to pale green
Form
Powder
Ash
? 3.0%
Description
Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent, Protein Standard, Diluent, Protein, Conjugate or Enzyme Stabilizer. Bovine Serum Albumin is applicable for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Frequently used as a blocking reagent; particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. Hybridization, Selected Cell Culture applications
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• Albumin bovine/fraction V is a serum albumin protein commonly used in laboratory experiments including, enzyme-linked immunosorbent assay (ELISAs), immunoblots and immune-histochemistry or -fluorescence
Applications
• Albumin bovine/fraction V displays blocking activity mainly when casein-sensitive antibodies, including phospho-specific antibodies, are used
• In genetic approaches this compound can be used to increase polymerase chain reactions (PCR) yields from low purity templates
• This compound is useful for stabilizing enzymes during storage and also for enzymatic reactions where the absence of nucleases is crucial
• In in vitro cultures, albumin bovine/fraction V has been applied as a nutrient in cell and microbial cultures especially when serum-free media is used
• It is commonly used as a protein standard to obtain calibration curves for several laboratorial experiments
• Albumin bovine/fraction V is a small protein commonly used in immunohistochemistry to block nonspecific regions. The binding of this compound to nonspecific binding sites increases the probability of the antibodies binds only to the region of interest.
RUO – Research Use Only
Figures
Documents & Downloads
Certificates
Search by lot number or partial lot number
Frequently asked questions (FAQs)
Citations & References
Search citations by name, author, journal title or abstract text