Thermo Scientific™
GeneJET Plasmid Miniprep Kit
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Description
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.Highlights
• Efficient—high yields of up to 20 µg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use
Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol
Related Products
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit
Resuspension Solution for GeneJET Plasmid Miniprep Kit
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| High Throughput Compatibility: | Compatible (Vacuum manifold) |
|---|---|
| Sample Type (General): | Bacteria |
| Downstream Application: | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Sequencing, Transformation |
| Product Line: | GeneJET |
| Product Size: | 250 preps |
