Biotin DecaLabel DNA Labeling Kit

Catalog number: K0651

Thermo Scientific™  Related applications: Nucleic Acid Labeling & Oligo Synthesis

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Description

Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

Highlights

Non-radioactive labeling of DNA
Efficient priming of labeling reactions with random decamers
High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Applications

• Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.

Principle

Random decamers are annealed to a denatured template DNA molecule, and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand.

Related Products
Biotin DecaLabel DNA Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 10 reactions
Final Product: DNA
Labeling Method: Direct Labeling
Labeling Target: DNA (General), PCR Products, Genomic DNA
Detection Method: Biotin-Based
Includes Label or Dye: Yes

Documents

Manuals & protocols