Thermo Scientific™

GeneJET Gel Extraction Kit

Catalog number:  K0691

Related applications:

DNA Extraction

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Description

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights

Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
Fast: procedure takes only 15 minutes
Convenient: spin columns are capped and assembled with collection tubes

Applications

• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
In situ hybridization
• Labeling
• Cloning
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR
Purification Target: DNA from Enzymatic Reactions (e.g. PCR)
Sample Type: Gel Samples
No. of Reactions: 50 preps
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: GeneJET