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Thermo Scientific™
GeneJET RNA Purification Kit
Catalog number: K0731
Thermo Scientific™
GeneJET RNA Purification Kit
Catalog number: K0731
Catalog Number
K0731
Unit Size
50 preps
Price (USD)
Price: 261.00
Your Price:
Availability
-
Quantity
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| K0731 | 50 preps | Price: 261.00 Your Price: | - | ||
| K0732 | 250 preps | Price: 1,076.00 Your Price: | - |
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Product Overview
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Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
• Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient – high yields of total RNA.
• Fast – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Convenient – spin columns are capped and assembled with collection tubes
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assayIncludes Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Collection Tubes) Collection Tubes (2 mL) Collection Tubes (1.5 mL) Detailed Protocol
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
• Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient – high yields of total RNA.
• Fast – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Convenient – spin columns are capped and assembled with collection tubes
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assayIncludes Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Collection Tubes) Collection Tubes (2 mL) Collection Tubes (1.5 mL) Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation Technology
Silica Spin Column
Purification Time
15 min. (After sample homogenization)
Elution Volume
50–100 μL
Sample Type
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
50 Preps
Scale
Mini
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤109 cells
Blood: 0.5 mL
Cells: ≤107
Tissue and Insects: 30 mg
Yeast: ≤4 x 108 cells
Enzymatic reactions: ≤100 μL
Blood: 0.5 mL
Cells: ≤107
Tissue and Insects: 30 mg
Yeast: ≤4 x 108 cells
Enzymatic reactions: ≤100 μL
Yield
Up to 160 μg
Contents & Storage
• 600 µL Proteinase K; store at room temperature until opened, -20°C after opening
• 40 mL Lysis Buffer; room temperature
• 40 mL Wash Buffer 1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature
• 40 mL Lysis Buffer; room temperature
• 40 mL Wash Buffer 1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature