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GeneJET RNA Purification Kit
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
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| Catalog Number | Quantity |
|---|---|
| K0731 | 50 Preps |
| K0732 | 250 Preps |
Catalog number K0731
Price (USD)/ Each
236.65
Online Exclusive
279.00Save 42.35 (15%)
Quantity:
50 Preps
Price (USD)/ Each
236.65
Online Exclusive
279.00Save 42.35 (15%)
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
- Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
- Efficient—high yields of total RNA
- Fast—15 minutes after lysis step
- Pure—an A260/280 ratio > 1.9
- Convenient—spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50–100 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time15 min. (After sample homogenization)
Quantity50 Preps
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells, Blood: 0.5 mL, Cells: ≤107, Tissue and Insects: 30 mg, Yeast: ≤4 x 108 cells, Enzymatic reactions: ≤100 μL
YieldUp to 160 μg
Isolation TechnologySilica Spin Column
Sample TypeBacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Unit SizeEach
Contents & Storage
• 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 40 mL Lysis Buffer; room temperature
• 40 mL Wash Buffer 1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature
• 40 mL Lysis Buffer; room temperature
• 40 mL Wash Buffer 1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature