Thermo Scientific™

DreamTaq PCR Master Mix (2X)

Catalog number:  K1071

Related applications:

PCR

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Description

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase

Highlights

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Incorporates modified nucleotides, but does not incorporate dUTP

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• High throughput PCR
• Genotyping
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Hot Start: No
Product Line: DreamTaq
Reaction Format: SuperMix or Master Mix
Reaction Speed: Standard
Polymerase: DreamTaq DNA Polymerase
Overhang: 3'-A

Contents & storage

Contains:
• 4 x 1.25 mL DreamTaq PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20°C.