This cell line is available as dividing cells by requesting a quote for K2186 from dividingcells@invitrogen.com

GeneBLAzer™ AVPR1A CHO-K1 DA(Division Arrested) cells and AVPR1A-NFAT-bla CHO-K1 cells contain the human Arginine Vasopressin 1a (AVPR1A) receptor stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the nuclear factor of activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both AVPR1A CHO-K1 DA cells and AVPR1A-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC₅₀ concentrations of vasopressin, (Figure 1). In addition, AVPR1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The family of receptors that recognize vasopressin and oxytocin consists of four members: vasopressin receptors 1a (AVPR1a), 1b (AVPR1b), and 2 (AVPR2), and the oxytocin (OT) receptor. AVPR1a, AVPR1b, and the OT receptor couple to the Gq/G11 class of G proteins and trigger the release of intracellular calcium when stimulated. AVPR2 receptors couple to Gs proteins and activate adenyl cyclase. All three AVPR receptors have high affinity for their endogenous ligand, vasopressin, but AVPR1b has less affinity for oxytocin than does AVPR1a or AVPR2. The hormones vasopressin and oxytocin are synthesized in the hypothalamus. Oxytocin causes contractions in the uterus during childbirth. The main functions of arginine vasopressin (AVP) are reabsorption of water by the kidneys and contraction of smooth muscle in arteries. This system may also play a role in memory and learning.


Storage:

GeneBLAzer™ AVPR1A CHO-K1 DA Cells and AVPR1A-NFAT-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ AVPR1A CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate.(Other materials are required separately; please refer to the protocol).
Includes:
•AVPR1A CHO-K1 DA cells (K1323A)
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ AVPR1A CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR1A CHO-K1 DA cells (K1324A)
•1 protocol
•Certificate of Analysis

GeneBLAzer™ AVPR1A-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR1A-NFAT-bla CHO-K1 cells (K1286)
•1 protocol
•Certificate of Analysis