Thermo Scientific™

MuSeek Library Preparation Kit, Ion Torrent compatible

Catalog number:  K1331

 Related applications:

Ion Torrent™ Next-Generation Sequencing

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Thermo Scientific MuSeek Library Preparation Kit for Ion Torrent™ is designed for generation of high‐quality genomic DNA libraries for sequencing with the Ion Personal Genome Machine™ (PGM™) and Ion Proton™ systems. The fast protocol utilizes MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adaptors are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 100 ng of the sample DNA the same protocol can be used to generate 100-400 bp insert libraries.


Fast—generates NGS fragment library in less than 80 minutes
Convenient —no physical shearing, end-repair and ligation steps
Easy to use—same protocol for generating different length DNA fragment libraries


• DNA fragment library preparation for downstream use in next generation sequencing workflow.

• MuSeek Enzyme Mix
• MuSeek Fragmentation Reaction Buffer
• MuSeek Stop Solution
• Control DNA
• MuSeek Adaptor Addition Primer Mix
• MuSeek Adaptor Addition Reaction Buffer
• Phusion Hot Start II High-Fidelity DNA Polymerase
• MuSeek Sequencing Primer

Note: The kit is shipped on dry ice. Upon arrival, open the kit and place MuSeek Enzyme Mix for storage below -70°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature, and the remaining kit components at -20°C.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Library Type: Fragment Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Line: MuSeek
Product Size: 10 reactions


Manuals & protocols