This cell line is available as dividing cells by requesting a quote for K1231 from dividingcells@invitrogen.com

GeneBLAzer™ CCKBR HEK 293T DA(Division Arrested) cells and CCKBR-NFAT-bla HEK 293T cells contain the human Cholecystokinin (CCKBR) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line. CellSensor™ NFAT-bla HEK 293T cells (Cat. no.K1179) contain a beta-lactamase reporter gene under control of the nuclear factor of activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both CCKBR HEK 293T DA cells and CCKBR-NFAT-bla HEK 293T cells are functionally validated for Z’-factor and EC₅₀ concentrations of cholecystokinin (CCK), (Figure 1). In addition, CCKBR-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). Cholecystokinin (CCK) is widely distributed throughout the CNS and digestive system. Through CCKAR and CCKBR, it is thought to play a role in many digestive processes as well as anxiety, depression, psychosis, cognition and nociception. Studies have shown CCKBR could be involved in anxiety, learning and memory, as well as growth of gastric mucosa.


Storage:

GeneBLAzer™ CCKBR HEK 293T DA Cells and CCKBR-NFAT-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ CCKBR HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•CCKBR HEK 293T DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ CCKBR HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•CCKBR HEK 293T DA cells
•1 protocol
•Certificate of Analysis

GeneBLAzer™ CCKBR-NFAT-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•CCKBR-NFAT-bla HEK 293T cells
•1 protocol
•Certificate of Analysis