GeneBLAzer® MR DA Assay Kit

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

GeneBLAzer®MR DA(Division Arrested) cells and MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NAbinding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-MR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both MR DA cells and MRUAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of aldosterone. In addition, MR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and substrate loading time. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Antagonists: LE 135
Gene Symbol: MR
Product Size: 1 plate
Primary Agonist: All-trans-retinoic Acid
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Alternate Agonists: AM 580, Fenretinide, TTNPB, 9-cis- retinoic acid, Ch 55

Contents & storage

Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Sufficient division-arrested cells and substrate to assay one 384-well plate. Includes: MR HEK 293T DA cells (K1409A) LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Documents

Manuals & protocols