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The GeneBLAzer® HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession # NM_001525) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.
The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Cell-based beta lactamase reporter gene
Pool of Stable Cells
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 cells o GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 Assay Protocol o Certificate of Analysis The GeneBLAzer™ HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.