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Thermo Scientific™

GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells

Catalog number:  K1446

Related applications:

Pharma & Biopharma

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Description

The GeneBLAzer® HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession # NM_001525) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.

The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: BC074796.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: HCRTR1
Population Status: Pool of Stable Cells
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: HCRTR1

Contents & storage

(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 cells
o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 Assay Protocol
o Certificate of Analysis
The GeneBLAzer® HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.