CellSensor® NFAT CHO-K1 DA Assay Kit

Catalog number: K1447

 Related applications: Target & Lead Identification & Validation

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Description

The CellSensor® NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Cell State: Division-Arrested Cells
Product Size: 1 plate
Druggable Target: GPCRs, Signaling Pathway

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o CellSensor® NFAT CHO-K1 DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o CellSensor® NFAT CHO-K1 Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The CellSensor® NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols