Thermo Scientific™

Tango™ H4-bla U2OS

Catalog number:  K1465

Related applications:

Pharma & Biopharma

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Description

The Tango™ H4-bla U2OS cells contain the human Histamine Receptor 4 (H4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ H4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of 4-Methylhistamine. In addition, Tango™ H4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_067637.2
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Division-Arrested Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: HRH4
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®, Tango™
Readout: End Point
Reporter Gene: BLA (Beta-Lactamase)
System Type: Tango™
Target Entry: HRH4, H4
Technique: FRET

Contents & storage

Contents:
K1465 Tango™ H4-bla U2OS cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ H4-bla U2OS cells
• Tango™ H4-bla U2OS Assay Protocol
• Certificate of Analysis

Storage:
The Tango™ H4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.