The Tango™ H4-bla U2OS cells contain the human Histamine Receptor 4 (H4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ H4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of 4-Methylhistamine. In addition, Tango™ H4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NP_067637.2
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
HRH4
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
HRH4, H4
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
Contents: K1465 Tango™ H4-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ H4-bla U2OS cells • Tango™ H4-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ H4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.