During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.
Thermo Scientific™

CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line

Catalog number:  K1468

Related applications:

Cellular Imaging

Back to top


The CellSensor® T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline- induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.


Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: HeLa
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Kinases
Product Line: CellSensor™, T-REx™
System Type: CellSensor™
Target Entry: IR, IGF1R, PI3K, AKT1, FOXO3, PI3K/AKT/FOXO3 pathway

Contents & storage

CellSensor® T-REx™ FOXO3 DBE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.