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Thermo Scientific™

Tango™ GPR8-bla U2OS Cells

Catalog number:  K1478

Related applications:

Pharma & Biopharma

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Description

The Tango™ GPR8-bla U2OS cells contain the human Neuropeptides B/W receptor 2 (GPR8) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR8-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Neuropeptide B-29 (NPB29). In addition, Tango™ GPR8-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_005286
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Dividing Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: GPR8
Population Status: Pool of Stable Cells
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Shipping Condition: Room Temperature
System Type: Tango™
Target Entry: GPR8

Contents & storage

(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
o Tango™ GPR8-bla U2OS cells
o Tango™ GPR8-bla U2OS Assay Protocol
o Certificate of Analysis
The Tango™ GPR8-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.