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The GeneBLAzer® CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), accession # NM_001742.2, stably integrated into the CellSensor® CRE-bla Freestyle293 cell line. The CellSensor® CRE-bla Freestyle293 cells contain a beta-lactamase (bla) reporter gene under control of the cAMP (CRE) response element.
CALCR-CRE-bla Freestyle293 cells have been tested for variable assay conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time and functionally validated for Z' and EC50 concentrations of calcitonin. Additional testing data using alternate stimuli are also provided. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Cell-based beta lactamase reporter gene
Single Cell Clone Origin
FreeStyle™ 293-F Cells
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells o GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F Assay Protocol o Certificate of Analysis The GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.