Thermo Scientific™

Tango™ HRH3-bla U2OS DA Assay Kit

Catalog number:  K1509

Related applications:

Pharma & Biopharma

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Description

The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine. In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_007232
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Division-Arrested Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: HRH3
Population Status: Pool of Stable Cells
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
System Type: Tango™
Target Entry: HRH3, H3

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o Tango™ HRH3-bla U2OS DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o Tango™ HRH3-bla U2OS Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.