Thermo Scientific™

Tango™ HRH3-bla U2OS DA Assay Kit

Catalog number: K1509
Thermo Scientific™

Tango™ HRH3-bla U2OS DA Assay Kit

Catalog number: K1509
Catalog Number
K1509
also known as 374078.0
Unit Size
1 plate
Price (USD)
902.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K1509
also known as 374078.0
1 plate
902.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine. In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_007232
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Division-Arrested Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Detection Method
Fluorescent
Gene Symbol
HRH3
Population Status
Pool of Stable Cells
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
HRH3, H3
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs

Contents & Storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o Tango™ HRH3-bla U2OS DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o Tango™ HRH3-bla U2OS Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Figures

Frequently asked questions (FAQs)

Citations & References