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Thermo Scientific™

CellSensor™ TrkA-NFAT-bla CHO-K1 Cell Line

Catalog number:  K1516

Related applications:

Pharma & Biopharma

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Description

Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death. Neurotrophin signaling also mediates neuronal higher-order activities, such as learning, memory, and behavior. Alterations in neurotrophin levels and their receptors have been implicated in neurodegenerative disorders such as Alzheimer's disease and Huntington's disease, as well as psychiatric disorders. Neurotrophins propagate their signal by activating multiple signaling pathways. One of the signaling pathways of NGF (the ligand for TrkA) activates phospholipase C, releasing DAG and IP3, increasing downstream intracellular calcium, and activating protein kinase C. This promotes the translocation of the transcription factor, nuclear factor of activated T-cells (NFAT), from the cytosol into the nucleus, resulting in NFAT-dependent transcription.

The CellSensor® TrkA-NFAT-bla CHO-K1 Cell Line was engineered by integrating the human TrkA expression plasmid into the genome of existing CellSensor® NFAT-bla CHO-K1 Cell Line, which is engineered to express beta-lactamase under the control of NFAT. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and in cryopreserved cells, and has been validated for Z´-factor and EC50 values under optimized conditions using NGF 2.5s.

Additional testing information using various small molecule inhibitors is also provided.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: PKC and calcium pathway, TRKA

Contents & storage

CellSensor® TrkA-NFAT-bla CHO-K1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate
use.