Coupling of GTP-binding to the T cell receptor (TCR) zeta-chain with TCR-mediated signal transduction.
AuthorsSancho J, Peter ME, Franco R, Danielian S, Kang JS, Fagard R, Woods J, Reed JC, Kamoun M, Terhorst C
JournalJ Immunol
PubMed ID8096851
The zeta-subunit of the TCR binds GTP and is a well characterized substrate for a TCR-activated tyrosine kinase. To examine the possible coupling of GTP-binding to zeta with TCR-mediated signal transduction, a mutant (termed J32-3.2) of the T cell line Jurkat (J32) was used. Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 ... More
Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity.
AuthorsPurcell DF, Martin MA
JournalJ Virol
PubMed ID8411338
Multiple RNA splicing sites exist within human immunodeficiency virus type 1 (HIV-1) genomic RNA, and these sites enable the synthesis of many mRNAs for each of several viral proteins. We evaluated the biological significance of the alternatively spliced mRNA species during productive HIV-1 infections of peripheral blood lymphocytes and human ... More
Processing of Pro-atrial Natriuretic Peptide by Corin in Cardiac Myocytes.
Authors Wu Faye; Yan Wei; Pan Junliang; Morser John; Wu Qingyu;
JournalJ Biol Chem
PubMed ID11884416
'Corin is a type II transmembrane serine protease abundantly expressed in the heart. In a previous study using transfected 293 cells, we showed that corin converted pro-atrial natriuretic peptide (pro-ANP) to atrial natriuretic peptide (ANP), suggesting that corin is likely the pro-ANP convertase. Because other serine proteases such as thrombin ... More
Identification and characterization of galectin-9, a novel beta- galactoside-binding mammalian lectin.
AuthorsWada J, Kanwar YS
JournalJ Biol Chem
PubMed ID9038233
'A 36-kDa beta-galactoside mammalian lectin protein, designated as galectin-9, was isolated from mouse embryonic kidney by using a degenerate primer polymerase chain reaction and cloning strategy. Its deduced amino acid sequence had the characteristic conserved sequence motif of galectins. Endogenous galectin-9, extracted from liver and thymus, as well as recombinant ... More
Alloantigen presentation by individual clones of mouse splenic macrophages. Selective expression of IL-1 alpha in response to CD8+ T cell-derived IFN-gamma defines the alloantigen-presenting phenotype.
AuthorsMcCormack JM, Askew D, Walker WS
JournalJ Immunol
PubMed ID8228220
'Approximately one-third of mouse splenic macrophage (M theta) progenitors yield progeny that constitutively present MHC class I alloantigen to naive T cells, a response that is restricted to CD8+ T cells and is elicited in a CD4+ Th cell-independent manner. In addition, both the alloantigen-presenting (alloAP+) and nonpresenting (alloAP-) M ... More
Keratinocyte-derived IL-7 serves as a growth factor for dendritic epidermal T cells in mice.
AuthorsMatsue H, Bergstresser PR, Takashima A
JournalJ Immunol
PubMed ID8245446
'Dendritic epidermal T cells (DETC) are CD3+, CD45+, CD4-, CD8-, TCR-V gamma 3/V delta 1+ T lymphocytes that reside in symbiosis with keratinocytes in mouse epidermis. To address mechanisms by which these cells survive and proliferate within the epidermal environment, we have tested the hypothesis that cytokines secreted by neighboring ... More
Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI.
AuthorsYeowell HN, Allen JD, Walker LC, Overstreet MA, Murad S, Thai S
JournalMatrix Biology
PubMed ID10686424
'This study describes the relative contribution of the 10 cysteine residues in lysyl hydroxylase 1 (LH1) to enzyme activity. We have identified a novel mutation of a 15-bp deletion in exon 11 in one LH1 allele, that codes for amino acids 367-371 (DLCRQ), in two unrelated compound heterozygous patients with ... More
Enhanced sensitivity to cholera toxin in ADP-ribosylarginine hydrolase-deficient mice.
AuthorsKato J, Zhu J, Liu C, Moss J,
JournalMol Cell Biol
PubMed ID17526733
'Cholera toxin (CT) produced by Vibrio cholerae causes the devastating diarrhea of cholera by catalyzing the ADP-ribosylation of the alpha subunit of the intestinal Gs protein (Gsalpha), leading to characteristic water and electrolyte losses. Mammalian cells contain ADP-ribosyltransferases similar to CT and an ADP-ribosyl(arginine)protein hydrolase (ADPRH), which cleaves the ADP-ribose-(arginine)protein ... More
A novel protein-tyrosine phosphatase related to the homotypically adhering kappa and mu receptors.
AuthorsCheng J, Wu K, Armanini M, O'Rourke N, Dowbenko D, Lasky LA
JournalJ Biol Chem
PubMed ID9054423
'Here we describe a novel member of the receptor-like protein-tyrosine phosphatases (PTPs) termed PTP lambda, which is homologous to the homotypically adherent PTPs kappa and mu. Murine PTP lambda contains MAM, IgG, fibronectin type III, and dual phosphatase domains. As has been demonstrated for PTPs kappa and mu, PTP lambda ... More
Beta -Secretase Cleavage of Alzheimer's Amyloid Precursor Protein by the Transmembrane Aspartic Protease BACE
AuthorsRobert Vassar, Brian D. Bennett, Safura Babu-Khan, Steve Kahn, Elizabeth A. Mendiaz, Paul Denis, David B. Teplow, Sandra Ross, Patricia Amarante, Richard Loeloff, Yi Luo, Seth Fisher, Janis Fuller, Steven Edenson, Jackson Lile, Mark A. Jarosinski, Anja Leona Biere, Eileen Curran, Teresa Burgess, Jean-Claude Louis, Frank Collins, James Treanor, Gary Rogers, and Martin Citron
JournalScience
PubMed ID10531052
'Cerebral deposition of amyloid peptide (A) is an early and critical feature of Alzheimer''s disease. Ageneration depends on proteolytic cleavage of the amyloid precursor protein (APP) by two unknown proteases: -secretase and -secretase. These proteases are prime therapeutic targets. A transmembrane aspartic proteasewith all the known characteristics of ... More
Myc downregulation by transforming growth factor beta required for activation of the p15(Ink4b) G(1) arrest pathway.
AuthorsWarner BJ, Blain SW, Seoane J, Massague J
JournalMol Cell Biol
PubMed ID10454538
'The antimitogenic action of transforming growth factor beta (TGF-beta) in epithelial cells involves cyclin-dependent kinase (cdk) inhibitory gene responses and downregulation of c-Myc expression. Although the cdk inhibitory responses are sufficient for G(1) arrest, enforced expression of c-Myc prevents G(1) arrest by TGF-beta. We investigated the basis of this antagonism ... More
Distinctive gene expression patterns in human mammary epithelial cells and breast cancers.
AuthorsPerou CM, Jeffrey SS, van de Rijn M, Rees CA, Eisen MB, Ross DT, Pergamenschikov A, Williams CF, Zhu SX, Lee JC, Lashkari D, Shalon D, Brown PO, Botstein D
JournalProc Natl Acad Sci U S A
PubMed ID10430922
'cDNA microarrays and a clustering algorithm were used to identify patterns of gene expression in human mammary epithelial cells growing in culture and in primary human breast tumors. Clusters of coexpressed genes identified through manipulations of mammary epithelial cells in vitro also showed consistent patterns of variation in expression among ... More
Cell type-specific integrin variants with alternative alpha chain cytoplasmic domains.
AuthorsTamura RN, Cooper HM, Collo G, Quaranta V
JournalProc Natl Acad Sci U S A
PubMed ID1946438
'The integrin heterodimers composed of the alpha 6 subunit with the beta 1 or beta 4 subunit (alpha 6 beta 1 and alpha 6 beta 4) are receptors for laminin and basement membrane components, respectively. The alpha 3 beta 1 integrin recognizes laminin, collagen, fibronectin, or epiligrin. We report the ... More
Mediation by a CREB Family Transcription Factor of NGF-Dependent Survival of Sympathetic Neurons
AuthorsAntonella Riccio, 1 Sohyun Ahn, 1 Christopher M. Davenport, 1 Julie A. Blendy, 2 David D. Ginty 1*
JournalScience
PubMed ID10600750
'Nerve growth factor (NGF) and other neurotrophins support survival of neurons through processes that are incompletely understood. The transcription factor CREB is a critical mediator ofNGF-dependent gene expression, but whether CREB family transcription factors regulate expression of genes that contribute to NGF-dependent survival of sympathetic neurons is unknown. CREB-mediated gene ... More
Silk properties determined by gland-specific expression of a spider fibroin gene family.
AuthorsGuerette PA, Ginzinger DG, Weber BH, Gosline JM
JournalScience
PubMed ID8600519
'Spiders produce a variety of silks that range from Lycra-like elastic fibers to Kevlar-like superfibers. A gene family from the spider Araneus diadematus was found to encode silk-forming proteins (fibroins) with different proportions of amorphous glycine-rich domains and crystal domains built from poly(alanine) and poly(glycine-alanine) repeat motifs. Spiders produce silks ... More
GlcNAc 2-epimerase can serve a catabolic role in sialic acid metabolism.
'Sialic acid is a major determinant of carbohydrate-receptor interactions in many systems pertinent to human health and disease. N-Acetylmannosamine (ManNAc) is the first committed intermediate in the sialic acid biosynthetic pathway; thus, the mechanisms that control intracellular ManNAc levels are important regulators of sialic acid production. UDP-GlcNAc 2-epimerase and GlcNAc ... More
Insulin-like growth factor expression in human cancer cell lines.
AuthorsQuinn KA, Treston AM, Unsworth EJ, Miller MJ, Vos M, Grimley C, Battey J, Mulshine JL, Cuttitta F
JournalJ Biol Chem
PubMed ID8626706
'The insulin-like growth factors (IGFs), IGF-I and IGF-II, are potent mitogens for human lung and other epithelial cancer cell lines. Previous studies in defined medium lacking added IGF or insulin suggest that an IGF-related ligand can act as an autocrine growth factor for many cancer cell lines through action via ... More
Molecular cloning and functional expression of 5-HT1E-like rat and human 5-hydroxytryptamine receptor genes.
AuthorsLovenberg TW, Erlander MG, Baron BM, Racke M, Slone AL, Siegel BW, Craft CM, Burns JE, Danielson PE, Sutcliffe JG
JournalProc Natl Acad Sci U S A
PubMed ID8384716
'Sequential polymerase chain reaction experiments were performed to amplify a unique sequence representing a guanine nucleotide-binding protein (G-protein)-coupled receptor from rat hypothalamic cDNA. Degenerate oligonucleotides corresponding to conserved amino acids from transmembrane domains III, V, and VI of known receptors [5-HT1A, 5- HT1C, and 5-HT2; 5-HT is serotonin (5-hydroxytryptamine)] were ... More
Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs.
AuthorsCsank C, Martindale DW
JournalJ Biol Chem
PubMed ID1371507
'We have determined the nucleotide sequence of a protozoan aminoacyl- tRNA synthetase. The isoleucyl-tRNA synthetase (ileRS) gene [ilsA; formerly cupC, Martindale, D. W., Martindale, H. M., and Bruns, P. J. (1986) Nucleic Acids Res. 14, 1341-1354] from the ciliate Tetrahymena thermophila was sequenced and found to have eight introns, four ... More
Human dystrophin expression corrects the myopathic phenotype in transgenic mdx mice.
AuthorsWells DJ, Wells KE, Walsh FS, Davies KE, Goldspink G, Love DR, Chan-Thomas P, Dunckley MG, Piper T, Dickson G
JournalHum Mol Genet
PubMed ID1301134
'Duchenne and the less severe Becker form of muscular dystrophy (DMD,BMD) result from genetic deficiency in the level and/or activity of the protein dystrophin. The recent availability of cDNA based minigenes encoding recombinant dystrophin polypeptides has raised the possibility of somatic gene transfer as a therapeutic approach to treat dystrophin ... More
Cloning, characterization, and expression of a cDNA encoding an inducible nitric oxide synthase from the human chondrocyte.
AuthorsCharles IG, Palmer RM, Hickery MS, Bayliss MT, Chubb AP, Hall VS, Moss DW, Moncada S
JournalProc Natl Acad Sci U S A
PubMed ID7504305
'Incubation of human articular chondrocytes with interleukin 1 beta results in the time-dependent expression of nitric oxide (NO) synthase. We report here the isolation of a cDNA clone which encodes a protein of 1153 amino acids with a molecular mass of 131,213 Da and a calculated isoelectric point of 7.9. ... More
Inositol 1,4,5-trisphosphate receptors: distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation.
AuthorsDanoff SK, Ferris CD, Donath C, Fischer GA, Munemitsu S, Ullrich A, Snyder SH, Ross CA
JournalProc Natl Acad Sci U S A
PubMed ID1849282
'We have identified two distinct transcripts of inositol 1,4,5- trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The ... More
Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones.
AuthorsLuo Y, Raible D, Raper JA
JournalCell
PubMed ID8402908
'Repulsive guidance cues can steer neuronal growth cones during development and prevent mature axons from regenerating. We have identified a 100 kd glycoprotein in the chick brain that is a good candidate for a repulsive cue. Since it induces the collapse and paralysis of neuronal growth cones in vitro, we ... More
In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells.
Authors Yang Lijun; Li Shiwu; Hatch Heather; Ahrens Kim; Cornelius Janet G; Petersen Bryon E; Peck Ammon B;
JournalProc Natl Acad Sci U S A
PubMed ID12048252
Although organ-specific stem cells possess plasticity that permit differentiation along new lineages, production of endocrine pancreas and insulin-secreting beta cells from adult nonpancreatic stem cells has not been demonstrated. We present evidence that highly purified adult rat hepatic oval ... More
A human milk factor susceptible to cathepsin D inhibitors enhances human immunodeficiency virus type 1 infectivity and allows virus entry into a mammary epithelial cell line.
AuthorsEl Messaoudi K, Thiry LF, Liesnard C, Van Tieghem N, Bollen A, Moguilevsky N.
JournalJ Virol
PubMed ID10623764
Human immunodeficiency virus type 1 (HIV-1) growth in lymphocyte cultures was increased when the virus inoculum was incubated in breast milk. The enhancing effect of milk was abolished by anti-cathepsin D antibody or by pepstatin A, a cathepsin D inhibitor. The cathepsin D-producing CD4-negative MCF7 mammary cells supported the growth ... More
The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1.
AuthorsReeder JC, Cowman AF, Davern KM, Beeson JG, Thompson JK, Rogerson SJ, Brown GV
JournalProc Natl Acad Sci U S A
PubMed ID10220443
Chondroitin sulfate A (CSA) is an important receptor for the sequestration of Plasmodium falciparum in the placenta, but the parasite ligand involved in adhesion has not previously been identified. Here we report the identification of a var gene transcribed in association with binding to CSA and present evidence that the ... More
Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress.
AuthorsCrabb BS, Cooke BM, Reeder JC, Waller RF, Caruana SR, Davern KM, Wickham ME, Brown GV, Coppel RL, Cowman AF
JournalCell
PubMed ID9108483
Knobs at the surface of erythrocytes infected with Plasmodium falciparum have been proposed to be important in adherence of these cells to the vascular endothelium. This structure contains the knob- associated histidine-rich protein (KAHRP) and the adhesion receptor P. falciparum erythrocyte membrane protein 1. We have disrupted the gene encoding ... More
Restricted heterogeneity in T-cell antigen receptor V beta gene usage in the lymph nodes and arthritic joints of mice.
AuthorsHaqqi TM, Anderson GD, Banerjee S, David CS
JournalProc Natl Acad Sci U S A
PubMed ID1311091
We have used PCR to study the expression of T-cell antigen receptor beta RNA containing particular variable region (V) elements from transcripts directly in the cells isolated from joints and lymph nodes of B10.Q mice (H-2q) immunized with chicken type II collagen. Our data show that the T cells present ... More
Lymphotoxin beta, a novel member of the TNF family that forms a heteromeric complex with lymphotoxin on the cell surface.
AuthorsBrowning JL, Ngam-ek A, Lawton P, DeMarinis J, Tizard R, Chow EP, Hession C, O'Brine-Greco B, Foley SF, Ware CF
JournalCell
PubMed ID7916655
The lymphokine tumor necrosis factor (TNF) has a well-defined role as an inducer of inflammatory responses; however, the function of the structurally related molecule lymphotoxin (LT alpha) is unknown. LT alpha is present on the surface of activated T, B, and LAK cells as a complex with a 33 kd ... More
E-box mutations in the RAPSN promoter region in eight cases with congenital myasthenic syndrome.
AuthorsOhno K, Sadeh M, Blatt I, Brengman JM, Engel AG,
JournalHum Mol Genet
PubMed ID12651869
Myogenic determination factors are basic helix-loop-helix proteins that govern specification and differentiation of muscle cells, and bind to the E-box consensus sequence CANNTG in promoter regions of muscle-specific genes. No E-box mutation has been reported to date. RAPSN encodes rapsyn, a 43 kDa postsynaptic peripheral membrane protein that clusters the ... More
Involvement of a bifunctional fatty-acyl desaturase in the biosynthesis of the silkmoth, Bombyx mori, sex pheromone.
The straight-chain C(10) to C(18) unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated C(16) or C(18) fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis of these pheromone components, various combinations of limited chain-shortening ... More
Expression cloning of new receptors used by simian and human immunodeficiency viruses.
AuthorsDeng HK, Unutmaz D, KewalRamani VN, Littman DR
JournalNature
PubMed ID9230441
Several members of the chemokine-receptor family serve, in conjunction with CD4, as receptors for the entry ofhuman immunodeficiency virus type I (HIV-1) into cells. The principal receptor for entry of macrophage-tropic(M-tropic) HIV-1 strains is CCR5, whereas that for T-cell-line-tropic (T-tropic) strains is CXCR4. Unlike HIV-1,infection with either M-tropic or T-tropic ... More
Pro-B-cell-specific transcription and proapoptotic function of protein kinase Ceta.
AuthorsMorrow TA, Muljo SA, Zhang J, Hardwick JM, Schlissel MS
JournalMol Cell Biol
PubMed ID10409750
Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Ceta (PKCeta). PKCeta transcripts were readily detected in pro-B cells but were absent in pre-B cells. Although both ... More
The pollen determinant of self-incompatibility in Brassica campestris.
AuthorsTakayama S, Shiba H, Iwano M, Shimosato H, Che FS, Kai N, Watanabe M, Suzuki G, Hinata K, Isogai A
JournalProc Natl Acad Sci U S A
PubMed ID10677556
Many flowering plants possess self-incompatibility (SI) systems that prevent inbreeding. In Brassica, SI is controlled by a single polymorphic locus, the S locus. Two highly polymorphic S locus genes, SLG (S locus glycoprotein) and SRK (S receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic ... More
Elastin is an essential determinant of arterial morphogenesis.
AuthorsLi DY, Brooke B, Davis EC, Mecham RP, Sorensen LK, Boak BB, Eichwald E, Keating MT
JournalNature
PubMed ID9607766
Elastin, the main component of the extracellular matrix of arteries, was thought to have a purely structural role. Disruption of elastin was believed to lead to dissection of arteries, but we showed that mutations in one allele encoding elastin cause a human disease in which arteries are blocked, namely, supravalvular ... More
An inhibitory fragment derived from protein kinase Cepsilon prevents enhancement of nerve growth factor responses by ethanol and phorbol esters.
We have studied nerve growth factor (NGF)-induced differentiation of PC12 cells to identify PKC isozymes important for neuronal differentiation. Previous work showed that tumor-promoting phorbol esters and ethanol enhance NGF-induced mitogen-activated protein (MAP) kinase activation and neurite outgrowth by a PKC-dependent mechanism. Ethanol also increases expression of PKCdelta and PKCepsilon, ... More
Polymorphism in the mouse Tap-1 gene. Association with abnormal CD8+ T cell development in the nonobese nondiabetic mouse.
AuthorsPearce RB, Trigler L, Svaasand EK, Peterson CM
JournalJ Immunol
PubMed ID8228229
Tap-1 and Tap-2 genes code for a heterodimeric peptide transporter required for the normal maturation and surface expression of class I molecules. Polymorphic variants of these MHC encoded genes occur in rats and humans. After failing to amplify a 3' polymerase chain reaction (PCR) product from thymic and splenic cDNA ... More
Mast cell expression of gelatinases A and B is regulated by kit ligand and TGF-beta.
AuthorsFang KC, Wolters PJ, Steinhoff M, Bidgol A, Blount JL, Caughey GH
JournalJ Immunol
PubMed ID10228034
Our prior work shows that cultured BR cells derived from dog mastocytomas secrete the 92-kDa proenzyme form of gelatinase B. We provided a possible link between mast cell activation and metalloproteinase-mediated matrix degradation by demonstrating that alpha-chymase, a serine protease released from secretory granules by degranulating mast cells, converts progelatinase ... More
Developmental gene expression and tissue distribution of the CHIP28 water-channel protein.
AuthorsBondy C, Chin E, Smith BL, Preston GM, Agre P
JournalProc Natl Acad Sci U S A
PubMed ID8506291
The CHIP28 water channel is a major component of red cell and renal tubule membranes; however, its ontogeny and tissue distribution remain undefined. Three patterns of expression were identified when CHIP28 mRNA was surveyed by in situ hybridization histochemistry in rats between embryonic day 14 and maturity. (i) CHIP28 mRNA ... More
Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation
AuthorsKawahara A, Wilm T, Solnica-Krezel L, Dawid IB
JournalProc Natl Acad Sci U S A
PubMed ID11050240
During zebrafish development, zygotic gene expression initiated at the midblastula transition converts maternal information on embryo polarity into a transcriptional read-out. Expression of a homeobox gene, vega1, is activated at midblastula transition in all blastomeres, but is down-regulated dorsally before gastrulation. Ubiquitous expression of vega1 is maintained in bozozok mutants, ... More
Different patterns of TCR transgene expression in single-positive and double-negative T cells. Evidence for separate pathways of T cell maturation.
AuthorsCheng L, Dejbakhsh-Jones S, Liblau R, Zeng D, Strober S
JournalJ Immunol
PubMed ID8621892
Two lines of transgenic mice were established using the TCR alpha (V alpha 4.4-J alpha 24)- and beta (V beta 9-D beta 1.1-J beta 2.1)-chain genes from a cloned CD4-CD8-alpha beta + (double-negative; DN) T cell line from BALB/c mice. The TCR genes were expressed in CD4+CD8- and CD4- CD8+ ... More
Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.
AuthorsGoswami S, Qasba P, Ghatpande S, Carleton S, Deshpande AK, Baig M, Siddiqui MA
JournalMol Cell Biol
PubMed ID8035795
In the present study, we have used single chicken blastoderms of defined early developmental stages, beginning with the prestreak stage, stage 1 (V. Hamburger and H. L. Hamilton, J. Morphol. 88:49-92, 1951), to analyze the onset of cardiac myogenesis by monitoring the appearance of selected cardiac muscle tissue-specific gene transcripts ... More
Human rheumatoid B-1a (CD5+ B) cells make somatically hypermutated high affinity IgM rheumatoid factors.
AuthorsMantovani L, Wilder RL, Casali P
JournalJ Immunol
PubMed ID7686945
To analyze the structure and formally ascertain the B-1a cellular origin of IgM rheumatoid factor (RF) autoantibodies, we generated 4 IgM RF mAb-producing cell lines using sorted (surface CD5+) B-1a cells from a patient with active rheumatoid arthritis. The RF mAb111, mAb112, mAb113, and mAb114 were monoreactive and displayed a ... More
Congenital myasthenic syndrome caused by prolonged acetylcholine receptor channel openings due to a mutation in the M2 domain of the epsilon subunit.
AuthorsOhno K, Hutchinson DO, Milone M, Brengman JM, Bouzat C, Sine SM, Engel AG
JournalProc Natl Acad Sci U S A
PubMed ID7531341
In a congenital myasthenic syndrome with a severe endplate myopathy, patch-clamp studies revealed markedly prolonged acetylcholine receptor (AChR) channel openings. Molecular genetic analysis of AChR subunit genes demonstrated a heterozygous adenosine-to-cytosine transversion at nucleotide 790 in exon 8 of the epsilon-subunit gene, predicting substitution of proline for threonine at codon ... More
Transcriptional regulation by lovastatin and 25-hydroxycholesterol in HepG2 cells and molecular cloning and expression of the cDNA for the human hepatic squalene synthase.
AuthorsJiang G, McKenzie TL, Conrad DG, Shechter I
JournalJ Biol Chem
PubMed ID7685352
Primers, based on the cDNA nucleotide sequences for rat hepatic squalene synthase (EC 2.5.1.21) (McKenzie, T.L., Jiang, G., Straubhaar, J.R., Conrad, D., and Shechter, I. (1992) J. Biol. Chem. 267, 21368- 21374), were synthesized and used for the amplification and sequencing of a 1672-base pair (bp) cDNA for the human ... More
The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells.
We attempted to isolate novel receptor tyrosine kinase, which may play a role in hematopoietic development by screening for expressed sequences with conserved tyrosine kinase catalytic domains. Among the known tyrosine kinases identified in this screen, we found a gene with characteristics of a receptor tyrosine kinase but unusual motifs ... More
Mice devoid of gamma-aminobutyrate type A receptor beta3 subunit have epilepsy, cleft palate, and hypersensitive behavior.
AuthorsHomanics GE, DeLorey TM, Firestone LL, Quinlan JJ, Handforth A, Harrison NL, Krasowski MD, Rick CE, Korpi ER, Makela R, Brilliant MH, Hagiwara N, Ferguson C, Snyder K, Olsen RW
JournalProc Natl Acad Sci U S A
PubMed ID9108119
gamma-Aminobutyric acid type A receptors (GABA(A)-Rs) mediate the bulk of rapid inhibitory synaptic transmission in the central nervous system. The beta3 subunit is an essential component of the GABA(A)-R in many brain regions, especially during development, and is implicated in several pathophysiologic processes. We examined mice harboring a beta3 gene ... More
Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum.
AuthorsLombroso PJ, Murdoch G, Lerner M
JournalProc Natl Acad Sci U S A
PubMed ID1714595
A cDNA clone encoding a neural-specific putative protein-tyrosine- phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) has been isolated from a rat striatal cDNA library. The deduced amino acid sequence predicts a protein of approximately 369 amino acids with a strong homology to other members of the family of protein-tyrosine- phosphatases. In vitro ... More
Ferritin is a developmentally regulated nuclear protein of avian corneal epithelial cells.
AuthorsCai CX, Birk DE, Linsenmayer TF
JournalJ Biol Chem
PubMed ID9139744
Previously, we generated monoclonal antibodies against chicken corneal cells (Zak, N. B., and Linsenmayer, T. F. (1983) Dev. Biol. 99, 373). We have now observed that one group of these antibodies reacts with a developmentally regulated component of corneal epithelial cell nuclei. This component is the heavy chain of ferritin, ... More
Differential accumulation of transcripts encoding protein kinase homologs in greening pea seedlings.
AuthorsLin X, Feng XH, Watson JC
JournalProc Natl Acad Sci U S A
PubMed ID1714582
Degenerate oligonucleotides, corresponding to conserved regions within the catalytic domain of known protein-serine/threonine kinases, were used as primers for the polymerase chain reaction to amplify cDNA synthesized from poly(A)+ RNA purified from the apical buds of 7-day- old pea seedlings. Five partial cDNAs were obtained and designated PsPK1 through PsPK5 ... More
Characterization of the interaction between P143 and LEF-3 from two different baculovirus species: Choristoneura fumiferana nucleopolyhedrovirus LEF-3 can complement Autographa californica nucleopolyhedrovirus LEF-3 in supporting DNA replication.
AuthorsChen T, Sahri D, Carstens EB,
JournalJ Virol
PubMed ID14671115
The baculovirus protein P143 is essential for viral DNA replication in vivo, likely as a DNA helicase. We have demonstrated that another viral protein, LEF-3, first described as a single-stranded DNA binding protein, is required for transporting P143 into the nuclei of insect cells. Both of these proteins, along with ... More
Differential distribution of Ca2+-activated K+ channel splice variants among hair cells along the tonotopic axis of the chick cochlea.
AuthorsNavaratnam DS, Bell TJ, Tu TD, Cohen EL, Oberholtzer JC
JournalNeuron
PubMed ID9390520
We have cloned from the receptor epithelium of the chick cochlea a family of alternatively spliced cDNAs derived from cslo, which encodes a Ca2+-activated K+ channel like those shown to help determine the resonant frequency of electrically tuned hair cells. Our results from PCRs using template RNAs from both tonotopically ... More
Cloning of a cysteine protease required for the molting of Onchocerca volvulus third stage larvae [published erratum appears in J Biol Chem 1997 Feb 14;272(7):4645]
AuthorsLustigman S, McKerrow JH, Shah K, Lui J, Huima T, Hough M, Brotman B
JournalJ Biol Chem
PubMed ID8939969
We have investigated the involvement of a cysteine protease in the development of Onchocerca volvulus fourth stage larvae (L4) by testing the effect of cysteine protease inhibitors on the survival of third stage larvae (L3), and the molting of L3 to L4 in vitro. When larvae were cultured in the ... More
A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells.
AuthorsBaier LJ, Bogardus C, Sacchettini JC
JournalJ Biol Chem
PubMed ID8631905
The human intestinal fatty acid binding protein (IFABP) binds long- chain fatty acids in vitro, but its intracellular function has remained speculative. A polymorphism in the gene that encodes IFABP results in an alanine (Ala54) to threonine (Thr54) substitution at codon 54 that alters the in vitro binding affinity of ... More
Cloning and characterization of chicken YB-1: regulation of expression in the liver.
AuthorsGrant CE, Deeley RG
JournalMol Cell Biol
PubMed ID8321222
A cDNA expression library constructed from day 9 embryonic liver was screened with a previously identified protein binding site in the flanking region of the liver-specific, estrogen-dependent avian apoVLDLII gene. Two of the clones isolated were shown to encode the chicken homolog of the Y-box binding protein, YB-1 (dbpb), which ... More
Up-regulation of the alpha2-macroglobulin signaling receptor on rheumatoid synovial fibroblasts.
AuthorsMisra UK, Gonzalez-Gronow M, Gawdi G, Pizzo SV
JournalJ Biol Chem
PubMed ID8995289
In the present study, we demonstrate that the alpha2-macroglobulin (alpha2M) signaling receptor is up-regulated on rheumatoid synovial fibroblasts. In rheumatoid cells, 125I-alpha2M-methylamine bound to two sites; namely, one of high affinity (Kd approximately 52 pM) and the second of lower affinity (Kd approximately 9.7 nM). In normal synovial fibroblasts only ... More
Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.
The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Vaccinia DNA topoisomerase I to develop ... More
Oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells.
AuthorsSugiura K, Su YQ, Diaz FJ, Pangas SA, Sharma S, Wigglesworth K, O'Brien MJ, Matzuk MM, Shimasaki S, Eppig JJ,
JournalDevelopment
PubMed ID17553902
Mammalian oocytes are deficient in their ability to carry out glycolysis. Therefore, the products of glycolysis that are necessary for oocyte development are provided to oocytes by companion cumulus cells. Mouse oocytes secrete paracrine factors that promote glycolysis in cumulus cells. The objective of this study was to identify paracrine ... More
Computer assisted cloning of human neutral alpha-glucosidase C (GANC): a new paralog in the glycosyl hydrolase gene family 31.
Authors Hirschhorn R; Huie M L; Kasper J S;
JournalProc Natl Acad Sci U S A
PubMed ID12370436
The exponential expansion of the publicly available human DNA sequence database has increasingly facilitated cloning by homology of genes for biochemically defined, functionally similar proteins. We hypothesized that an as-yet uncloned human alpha-glucosidase (human neutral alpha-glucosidase C or GANC) is a previously uncharacterized member of a paralogous human glycosyl hydrolase ... More
Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays.
Authors De Gregorio E; Spellman P T; Rubin G M; Lemaitre B;
JournalProc Natl Acad Sci U S A
PubMed ID11606746
To identify new Drosophila genes involved in the immune response, we monitored the gene expression profile of adult flies in response to microbial infection by using high-density oligonucleotide microarrays encompassing nearly the full Drosophila genome. Of 13,197 genes tested, we have characterized 230 induced and 170 repressed by microbial infection, ... More
Exploring the transcriptome of the malaria sporozoite stage.
Authors Kappe S H; Gardner M J; Brown S M; Ross J; Matuschewski K; Ribeiro J M; Adams J H; Quackenbush J; Cho J; Carucci D J; Hoffman S L; Nussenzweig V;
JournalProc Natl Acad Sci U S A
PubMed ID11493695
Most studies of gene expression in Plasmodium have been concerned with asexual and/or sexual erythrocytic stages. Identification and cloning of genes expressed in the preerythrocytic stages lag far behind. We have constructed a high quality cDNA library of the Plasmodium sporozoite stage by using the rodent malaria parasite P. yoelii, ... More
Bee Venom Phospholipase Inhibits Malaria Parasite Development in Transgenic Mosquitoes.
Malaria kills millions of people every year, and new control measures are urgently needed. The recent demonstration that (effector) genes can be introduced into the mosquito germ line to diminish their ability to transmit the malaria parasite offers new hope toward the fight of the disease (Ito, J., Ghosh, A., ... More
Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.
AuthorsYoshimoto H, Saltsman K, Gasch AP, Li HX, Ogawa N, Botstein D, Brown PO, Cyert MS.
JournalJ Biol Chem
PubMed ID12058033
In Saccharomyces cerevisiae, the Ca(2+)/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca(2+) and Na(+), and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca(2+) (200 mm) or ... More
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish.
Authors Nearing J; Betka M; Quinn S; Hentschel H; Elger M; Baum M; Bai M; Chattopadyhay N; Brown E M; Hebert S C; Harris H W;
JournalProc Natl Acad Sci U S A
PubMed ID12093923
To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of Ca(2+), Mg(2+), and ... More
NFAT4 movement in native smooth muscle. A role for differential Ca(2+) signaling.
AuthorsStevenson AS, Gomez MF, Hill-Eubanks DC, Nelson MT
JournalJ Biol Chem
PubMed ID11278965
The transcription factor NFAT (nuclear factor of activated T-cells) plays a central role in mediating Ca(2+)-dependent gene transcription in a variety of cell types. Sustained increases in intracellular calcium concentration ([Ca(2+)]i) are presumed to be required for NFAT dephosphorylation by the Ca(2+)/calmodulin-dependent protein calcineurin and its subsequent nuclear translocation. Here, ... More