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Invitrogen™

RiboMinus™ Transcriptome Isolation Kit, human/mouse

Catalog number: K155002
Invitrogen™

RiboMinus™ Transcriptome Isolation Kit, human/mouse

Catalog number: K155002
Catalog Number
K155002
also known as K1550-02
Unit Size
6 preps
Price (USD)
Price: 586.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K155002
also known as K1550-02
6 preps
Price: 586.00
Your price:
-
Product Overview
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RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.

The RiboMinus™ Transcriptome Isolation Kits enable:

Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Magnetic Bead
Purification Time
1.25 hr.
Sample Type
Total RNA
Final Product Type
Transcriptome RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
No. of Reactions
6 Preps
Product Line
RiboMinus™
Quantity
1
Starting Material Amount
Up to 10 μg total RNA
Target
Transcriptome RNA
System Type
RiboMinus
Yield
1 μg
Form
Magnetic Bead

Contents & Storage

The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer.

The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.

Store the isolation modules at 4°C, and the concentration module at room temperature.

All reagents are guaranteed stable for 6 months when properly stored.

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