RiboMinus™ Transcriptome Isolation Kit, yeast

Catalog number: K155003

Ambion™  Related applications: Microarray Analysis | RNA Extraction

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Description

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): RNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Real-Time Quantitative PCR (qPCR), Northern Blotting, Microarray Analysis
Format: Magnetic Bead
System: RiboMinus
Quantity: 1
Product Size: 6 preps
Final Product: Transcriptome RNA
Yield (up to): 1 µg
Purification Time: 1.25 hrs
Number of Reactions: 6 Preps
Isolation Technology: Magnetic Bead
Sample Type (Specific): Total RNA (Yeast)
Starting Material (Amount): Up to 10 µg total RNA

Contents & storage

The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

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