RiboMinus™ Transcriptome Isolation Kit, bacteria

Description

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

Figures

Specifications

Sample Type (General):	RNA
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	Microarray Analysis, Northern Blotting, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Final Product:	Transcriptome RNA
Format:	Magnetic Bead
Isolation Technology:	Magnetic Bead
Number of Reactions:	12 Reactions
Product Line:	RiboMinus™
Product Size:	12 preps
Purification Time:	1.25 hrs
Quantity:	1
Sample Type (Specific):	Total RNA (Bacterial)
Starting Material (Amount):	Up to 10 µg total RNA
System:	RiboMinus
Yield (up to):	1 µg

Contents & storage