RiboMinus™ Transcriptome Isolation Kit, bacteria
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Invitrogen™

RiboMinus™ Transcriptome Isolation Kit, bacteria

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomalRead more
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Catalog NumberQuantity
K15500412 preps
Catalog number K155004
Price (USD)/ Each
888.00
Add to cart
Quantity:
12 preps
Price (USD)/ Each
888.00
Add to cart
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeTranscriptome RNA
For Use With (Application)Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
FormMagnetic Bead
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineRiboMinus™
Purification Time1.25 hr.
Quantity12 preps
Starting Material AmountUp to 10 μg total RNA
System TypeRiboMinus
Yield1 μg
Isolation TechnologyMagnetic Bead
Sample TypeTotal RNA (Bacterial)
TargetTranscriptome RNA
Unit SizeEach
Contents & Storage
The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer.

The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.

Store the isolation modules at 4°C, and the concentration module at room temperature.

All reagents are guaranteed stable for 6 months when properly stored.