Invitrogen™

RiboMinus™ Transcriptome Isolation Kit, bacteria

Catalog number: K155004
Invitrogen™

RiboMinus™ Transcriptome Isolation Kit, bacteria

Catalog number: K155004
Catalog Number
K155004
Unit Size
12 preps
Price (USD)
Price: 712.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K15500412 preps
Price: 712.00
Your Price:
-
Product Overview
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RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Magnetic Bead
Isolation Technology
Magnetic Bead
Purification Time
1.25 hr.
Sample Type
Total RNA (Bacterial)
Final Product Type
Transcriptome RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
No. of Reactions
12
Product Line
RiboMinus™
Quantity
1
Starting Material Amount
Up to 10 µg total RNA
Target
Transcriptome RNA
System Type
RiboMinus
Yield
1 µg

Contents & Storage

The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.