MuSeek Index Set 1, Illumina compatible

Catalog number: K1551

Thermo Scientific™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

Thermo Scientific MuSeek Index Set 1, Illumina™ compatible, is designed for generation of indexed genomic DNA libraries for sequencing on the Illumina MiSeq™ and HiSeq™ instruments. The set provides 4 indexed P5 adapters and 6 MuSeek indexed P7 adapters. This enables pooling of up to 24 dual-indexed libraries and conducting multiplexed sequencing analysis. The MuSeek Index Set 1, in combination with MuSeek Library Preparation kit, Illumina™ compatible, allows fast and simple indexed library construction from 50 ng of sample DNA, eliminating the need for physical shearing, end conversion and adapter ligation steps.

Highlights

• Enables sequencing of up to 24 dual-indexed fragment libraries in a single sequencing run.

Applications

• Indexed DNA fragment library preparation for next generation sequencing.

Includes
• MuSeek Indices M501-4
• MuSeek Indices M701-6
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Illumina ® MiSeq, Illumina® HiSeq 2500
Library Type: Fragment Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 24 dual indices (4 x 6), 4 each

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o Tango™ CMKLR1-bla U2OS DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o Tango™ CMKLR1-bla U2OS Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The Tango™ CMKLR1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols