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The Tango™ CHRM2-bla U2OS cells contain the human Cholinergic muscarinic 2 receptor (CHRM2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ CHRM2-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Carbachol. In addition, Tango™ CHRM2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Cell-based beta-arrestin recruitment
Tail from GRPR GPCR
Single Cell Clone Origin
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o Tango™ CHRM2-bla U2OS cells o Tango™ CHRM2-bla U2OS Assay Protocol o Certificate of Analysis The Tango™ CHRM2-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.