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Invitrogen™

mRNA Catcher™ PLUS Purification Kit

Catalog number: K157002
Invitrogen™

mRNA Catcher™ PLUS Purification Kit

Catalog number: K157002
Catalog Number
K157002
Unit Size
96 preps
Price (USD)
Price: 440.00
Online price: 400.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
960 preps
Price (USD)
Price: 3,360.00
Online price: 3,050.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K15700296 preps
Price: 440.00
Online price: 400.65
Your price:
-
K157003960 preps
Price: 3,360.00
Online price: 3,050.65
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The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

Robust performance—high binding capacity per well (>80 ng/well)
Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Oligo dT Affinity
Purification Time
1.5 hr. (Including sample homogenization)
Elution Volume
80 μL
Sample Type
Cells, Tissue, Total RNA, Whole Blood
Final Product Type
mRNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Equipment)
Robotic Liquid Handling Systems
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Quantity
96 Preps
Shipping Condition
Room Temperature
Starting Material Amount
Cells: 100–106
Tissue: 4–40 mg
Total RNA: 0.1–100 μg
Whole Blood: 40 μL
Yield
>80 ng

Contents & Storage

• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C
• 4 mL 2X Lysis Buffer L20; room temperature
• 30 mL Wash Buffer W15; room temperature
• 4 mL Dilution Buffer DL1; room temperature
• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
• 100 μL 0.5M DTT; 4°C

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