Thermo Scientific™

GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

Catalog number: K1577
Thermo Scientific™

GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

Catalog number: K1577
Catalog Number
K1577
Unit Size
1 plate
Price (USD)
896.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K15771 plate
896.00
Your Price:
***
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The GeneBLAzer® ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_000680
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
ADRA1A
Population Status
Pool of Stable Cells
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
ADRA1A
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer™ ADRA1A CHO-K1DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer™ ADRA1A CHO-K1Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer™ ADRA1A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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