Thermo Scientific™

GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

Catalog number:  K1577

Related applications:

Pharma & Biopharma

Back to top

Description

The GeneBLAzer® ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000680
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: ADRA1A
Population Status: Pool of Stable Cells
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: ADRA1A

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer® ADRA1A CHO-K1DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer® ADRA1A CHO-K1Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer® ADRA1A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.