Tango™ HTR2A-bla U2OS DA Assay Kit

Catalog number: K1613

 Related applications: Target & Lead Identification & Validation

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Description

The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HTR2A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: Tango™
Readout: End Point
Reporter: BLA (Beta-Lactamase)
Cell Line: U2OS
Cell State: Division-Arrested Cells
Gene Symbol: HTR2A
Product Size: 1 kit
Protein Family: GPCR (G-Protein Coupled Receptors)
Accession Number: NM_000621.2
Cytoplasmic Tail: Tail from AVPR2 GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

K1613 Tango™ HTR2A-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• Tango™ HTR2A-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• Tango™ HTR2A-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ HTR2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols