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Thermo Scientific™

CellSensor™ SIE-bla ME-180 Cell Line

Catalog number:  K1648

Related applications:

Pharma & Biopharma

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Description

The CellSensor® SIE-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the Sis-Inducible Element (SIE) stably integrated into ME-180 cells. To construct this cell line, the SIE-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to Interleukin-6 (IL-6). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z' and EC50 concentrations of IL-6. The CellSensor® SIE-bla ME-180 cell line is responsive to IL-6, OSM and IFNγ and can be used to probe the JAK/STAT signaling pathway. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Druggable Target: Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: STAT1, STAT3, JAK/STAT (IFN gamma, IL-6/OSM) pathway, JAK2, JAK1

Contents & storage

CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.