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CellSensor™ NFκB-bla THP-1 Cell Line

Catalog number:  K1662

Related applications:

Pharma & Biopharma

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The CellSensor® NFκB-bla THP-1 cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B (NFκB) response element stably integrated into THP-1 cells. To obtain the cell line, the NFκB-bla construct was transduced into THP-1 cells by lentivirus. Subsequent flow cytometry was used to isolate cells responsive to Tumor Necrosis Factor alpha (TNFα). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, and EC50 concentration of TNF&alpha. The CellSensor® NFκB-bla THP-1 cell line is responsive to TNFα and can be used to probe NFκB signaling pathways, including those involved in apoptosis, viral defense, cancer, inflammation, and autoimmune disease. This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries. Candidate drugs can be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.


Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Druggable Target: Cytokines, Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway

Contents & storage

CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.