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Thermo Scientific™

GeneBLAzer™ RARα-UAS-bla HEK 293T Cells

Catalog number:  K1685

Related applications:

Pharma & Biopharma

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Description

RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK293T cell line. GeneBLAzer®UAS-bla HEK 293T cells (catalog#K1104) stably express a beta-lactamase reporter gene under the transcriptional control of a 7x Upstream Activator Sequence (UAS). Transcription from the 7xUAS is activated by the binding of the GAL4 transcription factor DNA-binding-domain (DBD). The GAL4-DBD is expressed as a fusion protein with the ligand binding domain (LBD) of RAR alpha. When an agonist binds to the LBD of the GAL4(DBD)-RAR alpha(LBD) fusion protein it translocates to the nucleus where it binds to the 7x UAS inducing transcription of betalactamase. RAR alpha-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time and have been validated for Z' and EC50 concentrations of all-trans retinoic acid. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: Tetrac, T4
Antagonists: 1-850
Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Primary Agonist: T3 Hormone
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: RARA, RAR alpha, NR1B1

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: TR alpha-UAS-bla HEK 293T cells (K1260)