During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.
Thermo Scientific™

GeneBLAzer™ RARα-UAS-bla HEK 293T Cells

Catalog number: K1685
Thermo Scientific™

GeneBLAzer™ RARα-UAS-bla HEK 293T Cells

Catalog number: K1685
Catalog Number
K1685
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K16852 x 106 cells
8,140.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK293T cell line. GeneBLAzer®UAS-bla HEK 293T cells (catalog#K1104) stably express a beta-lactamase reporter gene under the transcriptional control of a 7x Upstream Activator Sequence (UAS). Transcription from the 7xUAS is activated by the binding of the GAL4 transcription factor DNA-binding-domain (DBD). The GAL4-DBD is expressed as a fusion protein with the ligand binding domain (LBD) of RAR alpha. When an agonist binds to the LBD of the GAL4(DBD)-RAR alpha(LBD) fusion protein it translocates to the nucleus where it binds to the 7x UAS inducing transcription of betalactamase. RAR alpha-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time and have been validated for Z' and EC50 concentrations of all-trans retinoic acid. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Tetrac, T4
Antagonists
1-850
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Druggable Target
Nuclear Receptors
Primary Agonist
T3 Hormone
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
System Type
GeneBLAzer™
Target Entry
RARA, RAR alpha, NR1B1
Type
HEK 293T Cell

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: TR alpha-UAS-bla HEK 293T cells (K1260)

Figures

Frequently asked questions (FAQs)

Citations & References