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Thermo Scientific™

GeneBLAzer™ PPARδ-UAS-bla HEK 293T Cells

Catalog number: K1690
Thermo Scientific™

GeneBLAzer™ PPARδ-UAS-bla HEK 293T Cells

Catalog number: K1690
Catalog Number
K1690
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K16902 x 106 cells
8,140.00
Your Price:
***
Product Overview
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PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Estriol, Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Antagonists
RU486+1 nM E2, 4-OH-tam+1 nM E2, 4-OH-tamoxifen, Tamoxifen, Tam+1 nM E2, RU486
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Druggable Target
Nuclear Receptors
Primary Agonist
17-β-estradiol
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
System Type
GeneBLAzer™
Target Entry
PPAR delta, NR1C2, PPARD
Type
HEK 293T Cell

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: PPAR delta-UAS-bla HEK 293T cells (K1244)

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