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      GeneBLAzer™ PR-UAS-bla HEK 293T Cells

      Catalog number:  K1693

       Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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      Description

      PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of R5020. In addition, PR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Alternate Agonists: 22 (R) OH-Cholesterol, 24 (S) OH-Cholesterol, 24 (S), 25-Epoxycholesterol, GW 3965
      Cell State: Dividing Cells
      Detection Method: Fluorescent
      Druggable Target: Nuclear Receptors
      Primary Agonist: TO901317
      Product Line: GeneBLAzer®
      Product Size: 2 x 106 cells
      Reporter: BLA (Beta-Lactamase)
      System: GeneBLAzer™

      Contents & storage

      Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Contains: PR-UAS-bla HEK 293T cells (K1103)

      Documents

      Manuals & protocols