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Thermo Scientific™

GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells

Catalog number:  K1695

Related applications:

Pharma & Biopharma

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Description

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonists: none
Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Primary Agonist: 9-cis-retinoic acid
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: RAR beta, NR1B2, RARB

Contents & storage

GeneBLAzer® Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.